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F6377

Sigma-Aldrich

Fluorescein sodium salt

Fluorescein sodium salt

Powder

Synonym(s):

Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine

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About This Item

Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
518-47-8
Molecular Weight:
376.27
Colour Index Number:
45350
Beilstein:
3833041
EC Number:
208-253-0
MDL number:
MFCD00167039
UNSPSC Code:
12171500
PubChem Substance ID:
24894907
NACRES:
NA.47
Pricing and availability is not currently available.

Product Name

Fluorescein sodium salt, used as fluorescent tracer

Quality Level

200

form

powder

technique(s)

titration: suitable

color

orange to brown

pH

8.3 (20 °C, 10 g/L)

mp

>300 °C

solubility

H2O: 1 mg/mL

density

0.78 g/cm3 at 29.6 °C (85.3 °F at 699.7 hPa)

fluorescence

λex 460 nm; λem 515 nm(lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

InChI key

RGPLGPBQJOQFJS-UHFFFAOYSA-L

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General description

Fluorescein is a xanthene dye and is a common labeling agent in biology.[1]

Application

Fluorescein sodium salt has been used as a solute to study microvessel permeability.[2][3] It has been used as a test molecule to study blood barrier penetration.[4]
Water soluble fluorescent dye used in permeability and vascular perfusion studies due to its small molecular radius.[5] Sodium fluorescein is used as a fluorescent tracer to study blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability in rodent models.[6][7][8] The dye was used as a probe substrate in the study of organic anion transporting polypeptide (OATP) mediated drug transport in hepatocytes.[9] Sodium fluorescein was also used to monitor in situ solute transportation into bone through fluorescence recovery after photobleaching (FRAP).[10]

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

423.7 °F - Pensky-Martens closed cup

Flash Point(C)

217.6 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS2708
SHBS3018
0000240645
0000240645
0000182362

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Temperature dependence of electronic parameters of organic Schottky diode based on fluorescein sodium salt.
Yahia IS, et al.
Synthetic Metals, 161(9), 881-887 (2011)
P Kozler et al.
Physiological research, 52(5), 607-614 (2003-10-11)
The aim was to study the blood-brain permeability according to the distribution in the rat brain of Evans blue (EB) and sodium fluorescein (NaFl) administered by an intracarotid injection. Eighteen animals were divided into six groups according to the state
D Montermini et al.
The Journal of physiology, 543(Pt 3), 959-975 (2002-09-17)
The permeability, P(S), to sodium fluorescein (Stokes-Einstein radius = 0.45 nm) has been measured in single mesenteric capillaries of pithed frogs and anaesthetised rats as perfusion velocity, U, was varied over a range from 400 up to 2000-10,000 microm s(-1).
Tom De Bruyn et al.
Journal of pharmaceutical sciences, 100(11), 5018-5030 (2011-08-13)
The aim of this study was to characterize the in vitro hepatic uptake kinetics of sodium fluorescein (NaFluo) and identify the transporters involved. NaFluo exhibited saturable uptake kinetics in suspended rat and human hepatocytes as reflected by K(m) values of
Electrical conductivity and dielectric relaxation behavior of fluorescein sodium salt (FSS).
Mansour SA, et al.
Solid State Communications, 150(29), 1386-1391 (2010)
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Questions

1–4 of 4 Questions  

  1. What is the purity of Fluorescein sodium salt (CAS number 518-47-8, article number F6377-100G)?

    1 answer

    1. This purity of this product is not determined.

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  2. Can this product be autoclaved once dissolved? How long can a concentrated dissolved stock solution be stored for?

    1 answer

    1. The stability of Fluorescein under autoclave conditions has not been determined. Solutions have typically been filter sterilized. Long term solution stability has also not been evaluated. However, solutions of Fluorescein Diacetate are stable at -20°C in ethanol when protected from light. Similar stability would be expected with the sodium salt form.

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  3. Hello, I am interested in Fluorescein or Fluoresein sodium salt to perform angiography in the living mouse and would like to know if your product has a pharmacological grade. Which product is used for angiography?

    1 answer

    1. Unfortunately, a pharmacological grade Fluorescein or Fluorescein sodium salt is not available at this time.

      Helpful?

  4. Is F6377 soluble in ethanol?

    1 answer

    1. The substance is soluble in water (500 g/l) at 20°C and slightly soluble in ethanol, but insoluble in benzene, chloroform, and ether.

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