Several types of MRS broths exist, with most broths having pH values between 5.7 and 6.5. However, the pH of our MRS Broth modified, Vegitone, with catalog no. 38944, is indeed 4.3 as some strains of lactobacilli show reduced growth under higher pH values. For instance, Lactobacillus fermentum ATCC 9338 has been shown to grow well at pH 4.1 and Lactobacillus plantarum can grow at pH values as low as 3.4.
38944
MRS Broth modified, Vegitone
suitable for microbiology, NutriSelect® Plus
Synonym(s):
DeMan, Rogosa and Sharpe Agar modified, Vegitone, Vegitone MRS Broth modified
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About This Item
NACRES:
NA.85
UNSPSC Code:
41171607
Pricing and availability is not currently available.
sterility
non-sterile
Quality Segment
form
powder
shelf life
limited shelf life, expiry date on the label
composition
ammonium citrate, 2 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20 g/L , dipotassium phosphate, 2 g/L , magnesium sulfate, 0.10 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3 g/L , proteose peptone (vegetable), 10 g/L , sodium acetate, 5 g/L , yeast extract, 5 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
4.3±0.2 (25 °C)
application(s)
agriculture
bioburden testing
environmental
food and beverages, microbiology
suitability
selective by low pH for Bifidobacterium spp., selective by low pH for Lactobacillus spp.
Application
This MRS Broth contains plant peptone instead of animal peptone. It is recommended for the isolation and cultivation of Lactobacillus species.
Preparation Note
Suspend 47.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
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This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability selective by low pH for Bifidobacterium spp., selective by low pH for Lactobacillus spp. | suitability selective and differential for Lactobacillus spp., selective by low pH for Bifidobacterium spp. | suitability Actinomycetes spp., Streptomyces spp. | suitability selective by low pH for Lactobacillus spp. |
| composition ammonium citrate, 2 g/L , captan, 0.004 g/L , dipotassium phosphate, 2 g/L , manganese sulfate, 0.05 g/L , proteose peptone (vegetable), 10 g/L , yeast extract, 5 g/L , bromocresol green, 0.04 g/L , magnesium sulfate, 0.10 g/L , sodium acetate, 5 g/L , dextrose, 20 g/L , 2-phenylethyl alcohol, 3 g/L | composition agar, 15.0 g/L , ammonium citrate, 2.0 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20.0 g/L , dipotassium phosphate, 2.0 g/L , magnesium sulfate, 0.1 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3.0 g/L , proteose peptone (vegetable), 10.0 g/L , sodium acetate, 5.0 g/L , yeast extract, 5.0 g/L | composition ammonium sulfate, 1 g/L , calcium chloride, 0.02 g/L , L-cysteine hydrochloride, 1 g/L , dextrose, 5 g/L , magnesium sulfate, 0.2 g/L , monopotassium phosphate, 15 g/L , sodium chloride, 5 g/L , starch, soluble, 1 g/L , tryptone (vegetable), 4 g/L , tryptose (vegetable), 10 g/L , vegetable infusion powder, 10 g/L , yeast extract, 5 g/L | composition agar, 20 g/L , beef extract, 10 g/L , casein enzymic hydrolysate, 10 g/L , dextrose, 20 g/L , dipotassium phosphate, 2 g/L , polysorbate 80, 1 g/L , tomato juice, 2 g/L , yeast extract, 5 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) environmental |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type N95 (US)
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Regarding this culture medium (code 38944), you have said that pH should be adjusted to 4.3. I think there is a contraction here; the optimal pH of the three microorganisms that you tested in the technical data, that grew well in the range 5.5-6.5.
1 answer-
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Is this suitable for differentiating heterofermentative from homofermentative lactobacilli?By adjusting the pH the color changes from deep green to yellowish green, is this normal?What does the presence of green sediment at the end of all tubes indicate?
1 answer-
MRS broth is recommended for the isolation and cultivation of Lactobacillus species. However, it does not distinguish homofermentative from heterofermentative Lactobacilli. Specific medium needs to be used for this purpose as this broth allows growth of all Lactobacilli. The broth contains a dye, bromocresol green which allows a color change from deep green to yellowish green when the pH of the broth lowers. Presence of sediments could indicate incorrect dissolution of the dye.
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