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About This Item
UNSPSC Code:
12352203
NACRES:
NA.54
Conjugate:
unconjugated
Clone:
3F10, monoclonal
Application:
—
Citations:
41
Pricing and availability is not currently available.
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
lyophilized
packaging
pkg of 50 μg (11867423001), pkg of 500 μg (11867431001)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
General description
Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Application
Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.
Biochem/physiol Actions
Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
- Immunoprecipitation: 0.5 to 5 μg/ml
- Western blot: 50 to 200 ng/ml
Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.
Rehydrate for 10 min prior to use.
Analysis Note
Function tested in western blot.
Other Notes
For life science research only. Not for use in diagnostic procedures.
1 of 1
This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate peroxidase conjugate | conjugate biotin conjugate | conjugate unconjugated |
| biological source rat | biological source rat | biological source rat | biological source mouse |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| clone 3F10, monoclonal | clone clone 3F10, monoclonal | clone 3F10, monoclonal | clone 12CA5, monoclonal |
| form lyophilized | form lyophilized (clear, colorless solution after reconstitution) | form lyophilized (stabilized) | form frozen liquid (11666606001), lyophilized (11583816001) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
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signalword
Warning-Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11867431001 | 04061838251169 |
| 11867423001 | 04061838251152 |