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TMS-008

Alizarin-Red Staining Solution

Name: Alizarin-Red Staining Solution
Brand: MM

Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).

Synonym(s):

Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Level

100

concentration

40 mM in distilled water

technique(s)

cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable

Description

General description

Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research

Research Sub Category
Osteobiology

Arthritis

Physical form

Product is presented as a 40mM solution at pH 4.1 +/- 0.1.

Preparation Note

Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Analysis Note

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.

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Global Trade Item Number

SKU	GTIN
TMS-008-C	04053252914935