The volume per vial may vary from lot to lot, depending on the specific lot’s concentration. The current lot (4143790) is supplied at a concentration of 1 mg/mL, so 100 µg would be equivalent to a 100 µl-volume.
MAB3199
Anti-E-Cadherin Antibody, clone 67A4
clone 67A4, Chemicon®, from mouse
Synonym(s):
CD324
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
67A4, monoclonal
Application:
FACS, WB
Citations:
10
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
67A4, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
Immunogen
T-47D breast carcinoma line
Application
Anti-E-Cadherin Antibody, clone 67A4 detects level of E-Cadherin & has been published & validated for use in FC & WB.
Immunoblotting: 123kD in membrane fractions; MCF-7 membranes positive control. Confluent cells were washed three times with ice-cold PBS and lysed in 50mM HEPES pH 7.5, 150mM NaCl, 1mM EDTA, 10% glycerine, 1% triton X-100, 20mM Na(4)P(2)O(7), 2mM Na-vanadate, 100mM NaF, 2mM PMSF, 20μg/mL leupeptin, 1mM pNPP(para-nitrophenylphosphate), 20μg/mL aprotinin, and 200μM NH(4)-molybdate. Crude extracts were clarified by centrifugation (10,000 x g 10 min), and supernatants were taken for immunoprecipitation. Protein concentration of cleared lysates were determined by BCA protein assay. {Buhring, et al Luekemia 10:106-116, 1996}.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Original paper, Buhring, H et al Leukemia 10:106-116, 1996.
Optimal working dilutions must be determined by end user.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Original paper, Buhring, H et al Leukemia 10:106-116, 1996.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Biochem/physiol Actions
Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E-cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.
Antigen is expressed in non-neural epithelial tissues.
Antigen is expressed in non-neural epithelial tissues.
Physical form
Format: Purified
Protein A purified immunoglobulin. Liquid in 0.02M PBS with NaCl, pH=7.6, 0.1% Sodium azide.
Preparation Note
Maintain 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1103
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| biological source mouse | biological source mouse | biological source rabbit | biological source mouse |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| clone 67A4, monoclonal | clone 67A4, monoclonal | clone polyclonal | clone 67A4, monoclonal |
| Gene Information human ... CDH1(999) | Gene Information human ... CDH1(999) | Gene Information human ... CDH1(999) | Gene Information human ... CDH1(999) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Hello, what is the volume, or the concentration of this antibody: MAB3199 Anti-E-Cadherin Antibody, clone 67A4?
-
Helpful?
-
The current lot (4143790) & is supplied at a concentration of 1 mg/mL, so 100 µg would be equivalent to a 100 µl-volume. The volume per vial may vary from lot to lot, depending on the specific lot’s concentration.
Helpful?
-