MAB1273

Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1

clone 113-1, Chemicon^®, from mouse

• Synonym(s): Mitochondrial antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 113-1, monoclonal
• Species reactivity: human
• Application: IF, IHC (p), IP
• Citations: 205

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 113-1, monoclonal
• species reactivity: human
• should not react with: mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
• input: sample type neural stem cell(s)
• isotype: IgG1
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

65 kDa

This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.

Immunogen

Epitope: surface of intact mitochondria

Application

Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP.

Immunohistochemistry:
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.

Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium

The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.

Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.

MAB1273 recognizes a 65 kD protein by immunoprecipitation.

Optimal working dilutions and protocols must be determined by the end user.

Biochem/physiol Actions

Gives mitochondrial staining on all human cell types and does not cross react with rat and mouse tissue. The staining pattern is best described as a spaghetti-like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.

Reactivity with other species has not been determined

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant.

Preparation Note

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.

Analysis Note

Control
COS7 cells.

Routinely evaluated by immunohistochemistry on heart ventricle cells.

Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable