The sample amount depends on the detection method. The recommended sample amounts are as follows;
CBB staining: 25 µg or more
Fluorescent staining: 25 µg or less
Silver Staining: 10 µg or less
Fluorescent pre-labeling: 3 µg or less
BM-100
Auto2D® 2-D Electrophoresis Device
Automates 2D Gel Electrophoresis from 1st dimension IEF to 2nd dimension SDS-PAGE
Synonym(s):
2D Electrophoresis, 2DE, IEF, SDS-PAGE
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About This Item
NACRES:
NC.01
UNSPSC Code:
41100000
Pricing and availability is not currently available.
Product Name
Auto2D® 2-D Electrophoresis Device, automates 2D gel electrophoresis
product line
Auto2D®
technique(s)
SDS-PAGE: suitable, electrophoresis: suitable, western blot: suitable
storage temp.
room temp
General description
The Auto2D® 2-D Electrophoresis Device automates two-dimensional gel electrophoresis. The applied sample enters the first-dimension gel passively, separating proteins by their isoelectric points. After equilibration, the first-dimension gel is set onto a horizontal SDS-PAGE gel, which resolves proteins by molecular weight.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.
Application
- Differential proteomic analysis
- Post-translational modification
- Protein Isoforms
- Western Blotting
Features and Benefits
Auto2D® 2-D Electrophoresis Device is user-friendly and enable two-dimensional electrophoresis in 1-2 hours for faster, more reproducible results.
- Fully automated to eliminate tedious handling steps
- Fast results in 1-2 hours
- High reproducibility
- Easy to use, with no advanced training required
- Lower inter-operator variability
Preparation Note
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Legal Information
Auto2D is a registered trademark of Merck KGaA, Darmstadt, Germany
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| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. - |
| product line Auto2D® | product line Auto2D® | product line Auto2D® | product line Auto2D® |
| technique(s) SDS-PAGE: suitable, western blot: suitable, electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
Certificates of Analysis (COA)
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How much is the appropriate sample amount?
1 answer-
Helpful?
-
-
How much is the appropriate sample amount?
1 answer-
The sample amount depends on the detection method. The recommended sample amounts are as follows;
CBB staining: 25 µg or more
Fluorescent staining: 25 µg or less
Silver Staining: 10 µg or less
Fluorescent pre-labeling: 3 µg or lessHelpful?
-