ABT79

Anti-WASH complex subunit FAM21C Antibody

from rabbit

• Synonym(s): family with sequence similarity 21, member C, vaccinia virus penetration factor, WASH complex subunit FAM21C
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 12

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_056077
• UniProt accession no.: Q9Y4E1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WASHC4(23325)

Description

General description

FAM21C, also known as WASH complex subunit FAM21C, is a part of the a 500 kDa core complex that allows the Wiskott-Aldrich syndrome protein (WASH) function. FAM21C can be found in the membranes of early endosomes. FAM21C cooperates with CAPZ and hinders its actin-capping activity, links WASH to endosomes, and is required for WASH-dependent retromer-mediated sorting.

~145 kDa observed
Non-specific bands may be observed >200 kDa and at ~10 kDa

Immunogen

Recombinant protein corresponding to human WASH complex subunit FAM21C.

Application

Anti-WASH complex subunit FAM21C Antibody detects level of WASH complex subunit FAM21C & has been published & validated for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilutioin from a previous lot detected WASH complex subunit FAM21C in NIH/3T3, A431, and HeLa cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Physical form

Format: Purified

Protein G Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit FAM21C in 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1