ABT1385M

Anti-Glypican-1

serum, from rabbit

• Synonym(s): HSPG M12, GPC1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_110455
• UniProt accession no.: P35053
• target post-translational modification: unmodified
• Gene Information: rat ... Gpc1(58920)

Description

General description

Glypican-1 (UniProt: P35053; also known as GPC1, HSPG M12) is encoded by the Gpc1 gene (GeneID: 58920) in rat. Glypican-1 is attaches to the plasma membrane via glycosylphosphatidylinositol (GPI) anchors. It is a cell surface proteoglycan that bears heparan sulfate and may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Glypican-1 is required for proper skeletal muscle differentiation. It sequesters FGF2 in lipid rafts and blocks its binding to FGF receptors. Glypican-1 is specifically enriched on cancer-cell-derived exosomes and levels of glypican-1 positive exosomes correlate with tumor burden and the survival of pre- and post-surgical patients. Glypican-1 expression, both mRNA and protein, is upregulated in human pancreatic cancers cells and adjacent fibroblasts. (Ref.: Melo, SA et al. 2015. Nature. 523(7559):177-82; Kleeff, J et al. 1998. J. Clin. Invest. 102(9), 1662 1673).

Specificity

This rabbit polyclonal antibody detects Glypican-1 in Rat C6 glioma cells.

Immunogen

Epitope: unknown

GST-tagged recombinant fragment corresponding to the first 423 amino acids of rat Glypican-1.

Application

Anti-Glypican-1 Antibody, Cat. No. ABT1385 is a rabbit polyclonal antibody that detects Glypican-1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A representative lot detected Glypican-1 in rat brain homogenates and rat C6 glioma cells treated with heparitinase (Liang, Y., et. al. (1997). J Cell Biol. 139(4):851-64).

Immunocytochemistry Analysis: A representative lot detected Glypican-1 in rat C6 glioma cells (Liang, Y., et. al. (1997). J Cell Biol. 139(4):851-64).

Immunohistochemistry Analysis: A representative lot detected Glypican-1 in rat spinal cord and brain (Liang, Y., et. al. (1997). J Cell Biol. 139(4):851-64).

Quality

Evaluated by Immunocytochemistry in Rat C6 glioma cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Glypican-1 in Rat C6 glioma cells.

Target description

61.73 kDa calculated.

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1