ABE1791

Anti-Histone H3Q5ser

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Form: liquid

Properties

• antibody form: purified antibody
• clone: polyclonal
• form: liquid
• purified by: affinity chromatography
• species reactivity: rabbit, mouse, human
• concentration: (Please refer to lot specific datasheet.)
• technique(s): ChIP: suitable, dot blot: suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: Q16695

Description

General description

Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. It has been reported that Histone H3 can also undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5. (Ref.: Lepack, AE., et al (2020). Science. 368(6487); 197-201).

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal of human Histone H3.1 serotonylated at Glutamine 5.

Target molecular weight ~ 16 kDa observed. 15.508 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Epitope: N-terminus

Biochem/physiol Actions

Anti-Histone H3Q5ser is a rabbit polyclonal antibody that detects Histone H3 serotonylated at Glutamine 5. It targets an epitope within 10 amino acids from the N-terminal.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Recommended storage: +2°C to +8°C.

Analysis Note

Evaluated by dot blot with unmodified and Histone H3Q5-serotonyl peptides.

Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected Histone H3Q5-serotonyl peptide but not the unmodified Histone H3 control peptide (aa 1-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Other Notes

Human, Mouse.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.