AB9260

Anti-Ki-67 Antibody

Chemicon^®, from rabbit

• Synonym(s): Anti-Ki-67
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 261

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NM_002417.3
• UniProt accession no.: P46013
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MKI67(4288) ; mouse ... Mki67(17345) ; rat ... Mki67(291234)

Description

General description

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Predicted: 359 kDa MW may be observed at ~345 or 395 kDa

Immunogen

Synthetic peptide from human Ki-67

Application

Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.

Immunocytochemistry:
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).

Western Blot Analysis:
A previous lot of this antibody was used in western blot.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Ki-67

Physical form

Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.

Analysis Note

Control
Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.

Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2