AB5905

Anti-Vesicular Glutamate Transporter 1 Antibody

CHEMICON^®, guinea pig polyclonal

• Synonym(s): VGLUT1, BNPI
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat
• Application: IHC
• Citations: 617

Properties

• Product Name: Anti-Vesicular Glutamate Transporter 1 Antibody, serum, Chemicon^®
• biological source: guinea pig
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable
• NCBI accession no.: NP_446311
• UniProt accession no.: Q62634
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: rat ... Slc17A7(116638)

Description

General description

62 kDa

Vesicular glutamate transporter 1 (VGLUT1) is encoded by the gene mapped to human chromosome 19q13. VGLUT1 is specifically expressed in neuronal cells in the brain. It is characterized with a conserved C-terminal dileucine-like motif and two polyproline domains distal to C- terminal end, including one that binds to endocytic BAR (Bin/Amphiphysin/Rvs) domain protein, endophilin.

Immunogen

Synthetic peptide from rat VGLUT1 protein with no overlap to VGLUT2. The immunogen is available as AG208.

Application

• Tested Applications
  
  Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 1 in rat E18 cortical cells.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Recognizes Vesicular Glutamate Transporter 1 (VGLUT1, BNPI {Brain specific Na+ dependent inorganic phosphate cotransporter}). The antiserum has been tested on tissue sections from the rat central nervous system (CNS) using immunofluorescence histochemistry. The antiserum mainly labels nerve fibers and terminals. VGLUT1 is an excellent marker for glutamatergic nerve terminals. The staining pattern for the staining obtained with the VGLUT1 antiserum corresponds to the pattern described using other antisera to VGLUT1 (Bellocchio et al., 1998; Fremeau et al., 2001; Fujiyama et al., 2001; Sakata-Haga et al., 2001; Tong et al., 2001; Kaneko et al. 2002; Varoqui et al., 2002). Preabsorption of the VGLUT1 antiserum with immunogen peptide eliminates all immunostaining.

Physical form

Guinea pig Serum in 0.1% Sodium Azide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1