596200

Thioflavin T

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils.

• Synonym(s): Thioflavin T, ThT, Thioflavine T, Basic Yellow 1
• Empirical Formula (Hill Notation): C₁₇H₁₉ClN₂S
• CAS Number: 2390-54-7
• Molecular Weight: 318.86
• UNSPSC Code: 12171500
• NACRES: NA.25
• MDL number: MFCD00011944

Properties

• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• solubility: water: 5 mg/mL
• fluorescence: λ_ex ~385 nm (free), λ_ex ~437 nm, λ_em ~445 nm (free), λ_em ~485 nm
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: S1c2c(ccc(c2)C)N(C1=C3C=CC(=[N+](C)C)C=C3)C.[Cl-]
• InChI: 1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
• InChI key: JADVWWSKYZXRGX-UHFFFAOYSA-M

Description

General description

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils. Useful in monitoring stacked β sheet aggregates. Associates with aggregated fibrils of synthetic β/A4-derived peptides β(1-28) and β(1-40), resulting in a shift in the excitation maximum and an enhanced emission. Excitation max.: ~385 nm (free); ~437 nm (bound); emission max.: ~445 nm (free); ~485 nm (bound).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Exhibits enhanced fluorescence upon binding to amyloid fibrils

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Tjernberg, L.O., et al. 1999. J. Biol. Chem.274, 12619.
Naiki, H. and Nakakuki, K. 1996. Lab. Invest.74, 374.
LeVine, H. 1993. Protein Sci.2, 404.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS06,GHS05,GHS09
• signalword: Danger
• hcodes: H301,H317,H318,H410
• pcodes: P261 - P273 - P280 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable