550701-M

Pyruvate Oxidase, Aerococcus viridans

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

• Synonym(s): Pyruvate Oxidase, Aerococcus viridans
• CAS Number: 9001-96-1
• EC Number: 1.2.3.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00132148
• Specific activity: ≥25 U/mg

Properties

• Quality Segment: 100
• form: lyophilized solid
• specific activity: ≥25 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• impurities: ≤0.006% GPT
• color: yellow
• pI: 4.0
• solubility: aqueous buffer: soluble
• foreign activity: GOT and lactate oxidase ≤0.002%, catalase ≤0.3%
• storage temp.: −20°C

Description

General description

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hager, L.P. and Lipmann, F. 1955. Methods Enzymol. 1, 482.

One unit is defined as the amount of enzyme which generates 1 µmol of H₂O₂ per min at 37°C, pH 6.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable