480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

• Synonym(s): α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase
• CAS Number: 9001-67-6
• EC Number: 3.2.1.18 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• MDL number: MFCD00149915
• Specific activity: ≥10 units/mL, ≥250 units/mg protein
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 100
• description: RTECS - QQ3450000
• product line: EMPROVE^® EXPERT
• form: liquid
• specific activity: ≥10 units/mL, ≥250 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Note: 1 mU = 1 milliunit.

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS05,GHS07
• signalword: Warning
• pcodes: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• hcodes: H290,H315,H319,H335
• Storage Class: 12 - Non Combustible Liquids