475951

A22 hydrochloride

≥95% (HPLC),cell-permeable isothiourea compound, solid

• Synonym(s): MreB Perturbing Compound A22, S-(3,4-Dichlorobenzyl)isothiourea, HCl
• Empirical Formula (Hill Notation): C₈H₈Cl₂N₂S · xHCl
• CAS Number: 22816-60-0
• Molecular Weight: 235.13 (free base basis)
• UNSPSC Code: 51111800
• NACRES: NA.77
• MDL number: MFCD00035050
• Assay: ≥95% (HPLC)
• Form: solid
• Storage condition: OK to freeze, desiccated (hygroscopic), protect from light

Properties

• Product Name: MreB Perturbing Compound A22, A cell-permeable isothiourea compound that specifically, rapidly and reversibly perturbs MreB function without affecting eukaryotic actin polymerization.
• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• color: white
• solubility: water: 2 mg/mL, DMSO: 200 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: S(Cc1cc(c(cc1)Cl)Cl)\C(=N\[H])\N.Cl
• InChI: 1S/C8H8Cl2N2S.ClH/c9-6-2-1-5(3-7(6)10)4-13-8(11)12;/h1-3H,4H2,(H3,11,12);1H
• InChI key: VBJNMXMOMSWRDV-UHFFFAOYSA-N

Description

General description

A cell-permeable isothiourea compound that specifically, rapidly and reversibly perturbs MreB function without affecting eukaryotic actin polymerization. Reported to affect the growth and morphology of Caulobacter crescentus, convert rod-shaped E. coli to round cells, and block DNA segregation without affecting DNA replication. Suggested to interact with MreB′s nucleotide binding pocket and is useful for the temporal analysis of MreB function.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
MreB

Product does not compete with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution in DMSO, aliquot and freeze (-20°C. DMSO stock solutions are stable for up to 1 month at -20°C. Reconstitute in H₂O just prior to use; aqueous stock solutions are stable for up to 12 hr. Stable for up to 2 days in acidic medium.

Other Notes

Gitai, Z., et al. 2005. Cell120, 329.
Jensen, R.B., et al. 2002. Nat. Rev. Mol. Cell Biol.3, 167.
Iwai, N., et al. 2002. Biosci. Biotechnol. Biochem.66, 2658.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable