220477-M

Chitosanase, Streptomyces sp. N174

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

• Synonym(s): Chitosanase, Streptomyces sp. N174, Chitosan N-acetylglucosaminohydrolase
• CAS Number: 51570-20-8
• EC Number: 3.2.1.132 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• MDL number: MFCD00211797
• NACRES: NA.32
• Specific activity: ≥25 units/mg protein

Properties

• Quality Segment: 100
• form: liquid
• specific activity: ≥25 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• pI: 7.5 (temperature optimum 65°C for 10 minutes; recommended temperature 37°C)
• foreign activity: chitinolytic ≤0.01%, proteases <0.25 Azocasein U/ml
• storage temp.: −20°C

Description

General description

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

Native Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.

Physical form

In 50 M sodium acetate, 50% glycerol, pH 5.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein

One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1