1.37159

DL-Dithiothreitol

EMPROVE^® ESSENTIAL

• Synonym(s): DL-Dithiothreitol, Cleland’s reagent, DTT, threo-1,4-Dimercapto-2,3-butanediol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• EC Index Number: 222-468-7
• UNSPSC Code: 12352200
• Beilstein/REAXYS Number: 1719757
• MDL number: MFCD00004877

Properties

• biological source: synthetic
• Quality Segment: 500
• product line: EMPROVE^® ESSENTIAL
• technique(s): in vitro transcription (IVT): suitable
• mp: 41-44 °C (lit.)
• application(s): pharma/biopharma processes
• storage temp.: −20°C (−15°C to −25°C)
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

General description

Our SAFC Pharma^® portfolio of high-quality products for biopharmaceutical processing withstands strict quality control procedures and is produced according to MQ-500 requirements as defined by the M-Clarity program.
M-Clarity Program
As part of our Emprove^® Program, our raw materials are offered with Emprove^® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.
Our comprehensive portfolio of downstream process chemicals not only provides biopharmaceutical manufacturers with high-quality raw materials for production of classical and novel therapies, but also helps them get to market faster and simplify regulatory challenges. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DTT is an excellent reagent chemical used to reduce disulfide bonds commonly found in bioprocessing. DTT is used in buffer formulations to maintain and stabilize free -SH in applications such as protein based biotherapeutics and enzyme stability.

Packaging

1.37159.0010 / 10 g / Amber glass bottle
1.37159.0100 / 100 g / Amber glass bottle
1.37159.1000 / 1 kg / LDPE bag with outer HDPE drum
1.37159.9010 / 10 kg / LDPE bag with outer HDPE drum

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFC Pharma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• pcodes: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• hcodes: H302,H315,H318
• wgk: WGK 3