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FLAA

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

for ATP quantitation

Synonym(s):

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

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About This Item

EC Number:
UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.26

storage temp.

−20°C

General description

The Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of ATP in samples. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin. When ATP is the limiting reagent, the light emitted is proportional to the ATP present in the sample.

Application

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit has also been used in the quantification of ATP in 3D matrixes of human neurons, hepatic cells with ischemia, various bacterial cultures and lysosomes.

Kit Components Only

Product No.
Description

  • FL-AAB Dilution buffer 1 mL/vial

  • FL-AAM Assay mix 1 mL/vial

  • FL-AAS ATP standard 1 mL/vial

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Marco A Tavares et al.
Environmental toxicology and pharmacology, 40(1), 206-214 (2015-07-06)
Fipronil is an insecticide extensively used to control pests in crops and animals. There are relates of poisoning due to exposure of fipronil in mammals and the liver has been suggested as potential target. In this study, we evaluated the
Xi Zoë Zhong et al.
The Journal of physiology, 594(15), 4253-4266 (2016-08-02)
SLC17A9 proteins function as a lysosomal ATP transporter responsible for lysosomal ATP accumulation. P2X4 receptors act as lysosomal ion channels activated by luminal ATP. SLC17A9-mediated ATP transport across the lysosomal membrane is suppressed by Bafilomycin A1, the V-ATPase inhibitor. SLC17A9
John F C Steele et al.
PloS one, 14(8), e0221226-e0221226 (2019-08-29)
Plant NLRs are modular immune receptors that trigger rapid cell death in response to attempted infection by pathogens. A highly conserved nucleotide-binding domain shared with APAF-1, various R-proteins and CED-4 (NB-ARC domain) is proposed to act as a molecular switch
F Chen et al.
Journal of clinical microbiology, 32(11), 2791-2800 (1994-11-01)
A bioluminescent assay which employs the luciferin-luciferase ATP-dependent reaction was used to evaluate the viability of populations of Pneumocystis carinii derived from infected rat lungs. Contamination with host cells was reduced by a purification method which involved a combination of
Yuri L Boteon et al.
PloS one, 14(10), e0224066-e0224066 (2019-10-24)
The combination of hypothermic and normothermic machine perfusion (HMP+NMP) of the liver provides individual benefits of both techniques, improving the rescue of marginal organs. The aim of this study was to investigate the effect on the bioenergetic status and the

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