E9502

EPPS

≥99.5% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS
• Empirical Formula (Hill Notation): C₉H₂₀N₂O₄S
• CAS Number: 16052-06-5
• Molecular Weight: 252.33
• Beilstein: 3957385
• EC Number: 240-198-8
• MDL number: MFCD00006160
• UNSPSC Code: 12161700
• PubChem Substance ID: 24894698
• NACRES: NA.25

Properties

• Quality Level: 400
• Assay: ≥99.5% (titration)
• form: powder
• pH: 7.3-8.7
• useful pH range: 7.3-8.7
• pKa (25 °C): 8.0
• pKa: 8
• mp: 237-239 °C (lit.)
• solubility: H₂O: 25 g + 50 mL, clear, colorless
• λmax: A290 at ≤0.1 (C=33% w/w)
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCCS(O)(=O)=O)CC1
• InChI: 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)
• InChI key: OWXMKDGYPWMGEB-UHFFFAOYSA-N

Description

Application

EPPS has been used:

• to verify the effect of pH variation on the proteolytic activity of the posterior midgut (PMG) contents and anterior midgut (AMG) tissue homogenate
• to prepare a destaining buffer to destain intensely stained gel pieces
• to prepare digestion buffer for in-gel enzymatic digestion
• to reconstitute the digested peptides for the processing of brain-derived extracellular vesicle (EV) proteins and 16-plex isobaric tandem mass tag (TMT) peptide labeling

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves