D6421

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
• MDL number: MFCD00217417
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: sodium pyruvate: 0.055 g/L; L-glutamine: no; phenol red: yes; NaHCO₃: yes; HEPES: 15 mM; glucose: 3.15 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.

Application

DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Suitable for growth of wide range of cell types in low serum conditions.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.

Reconstitution

Supplement with 0.365 g/L L-glutamine.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable