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Sigma-Aldrich

JumpStart™ AccuTaq™ LA DNA Polymerase

Hot-start high fidelity Taq enzyme, 10X buffer included

Synonym(s):

JumpStart® AccuTaq™ LA DNA Polymerase

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About This Item

UNSPSC Code:
41106314
NACRES:
NA.55

Quality Level

200

form

liquid

usage

sufficient for 125 reactions
 sufficient for 500 reactions

feature

Long & Accurate PCR
dNTPs included: no
hotstart

concentration

2.5 unit/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR

shipped in

wet ice

storage temp.

−20°C

General description

JumpStart™ AccuTaq™ LA DNA Polymerase is a combination of Sigma′s high-fidelity blend, AccuTaq long and accurate (LA) DNA Polymerase, and the Taq neutralizing JumpStart Taq antibody. This specially formulated enzyme is the best choice for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot-start mechanism provides increased specificity and higher target yield in comparison to standard amplification.

Application

JumpStart™ AccuTaq™ LA DNA Polymerase has been used:
  • in polymerase chain reaction (PCR) for the amplification of soil DNA samples
  • in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs)
  • as a component of the PCR reaction mix for amplification of viral DNA through conventional PCR

Features and Benefits

  • JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
  • Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
  • Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

Packaging

Supplied with 10× reaction buffer.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
Jacobs is a registered trademark of Jacobs
JumpStart is a trademark of Sigma-Aldrich Co. LLC

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pier-Luc Tremblay et al.
mBio, 4(1), e00406-e00412 (2012-12-28)
It has been predicted that the Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD(+) oxidoreductase which contributes to ATP synthesis by an H(+)-translocating ATPase under both autotrophic and heterotrophic growth conditions. The recent development of methods for genetic manipulation
Transcriptional and post-transcriptional regulation of histone variant H2A. Z during sea urchin development
Hajdu M, et al.
Development, Growth & Differentiation, 58(9), 727-740 (2016)
Expression of Na+-dependent citrate transport in a strongly metastatic human prostate cancer PC-3M cell line: regulation by voltage-gated Na+ channel activity.
Mycielska, M.E., et al.
The Journal of Physiology, 253.2, 393-393 (2005)
Maja Łebska et al.
The Journal of biological chemistry, 285(9), 6217-6226 (2009-12-19)
Maize eukaryotic translation initiation factor 5A (ZmeIF5A) co-purifies with the catalytic alpha subunit of protein kinase CK2 and is phosphorylated by this enzyme. Phosphorylated ZmeIF5A was also identified after separation of maize leaf proteins by two-dimensional electrophoresis. Multiple sequence alignment
Amber L Mosley et al.
The Journal of biological chemistry, 278(22), 19660-19666 (2003-04-01)
Induction of insulin gene expression in response to high blood glucose levels is essential for maintaining glucose homeostasis. Although several transcription factors including Beta-2, Ribe3b1, and Pdx-1 have been shown to play a role in glucose stimulation of insulin gene
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Articles

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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