D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Synonym(s):
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
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recombinant
expressed in E. coli
Quality Segment
form
solid
specific activity
>=30 units/mg protein (biuret)
storage temp.
−20°C
General description
Research area: Cancer
Application
Diaphorase from Clostridium kluyveri has been used:
- in lactate dehydrogenase assay in gill cells.
- impregnated on the strip for formate dehydrogenase assay.
- in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
- for semiquinone generation during benzoquinone reduction.
- for evaluation of glucose uptake in cultured muscle cells.
- in lactate dehydrogenase cytotoxicity assay.
Biochem/physiol Actions
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
Physical form
Supplied as a lyophilized powder containing trehalose.
Other Notes
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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