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Dulbecco′s Modified Eagle′s Medium - high glucose

Name: Dulbecco′s Modified Eagle′s Medium - high glucose
Brand: SIGMA

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO₃: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 17.3 grams of powder per liter of medium.

Supplement with 3.7 g/L sodium bicarbonate.

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This Item	D7777	D5648	D6171
Sigma-Aldrich D1152 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D7777 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D5648 Dulbecco′s Modified Eagle′s Medium - high glucose	SAFC D6171 Dulbecco′s Modified Eagle′s Medium - high glucose
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form powder	form liquid
components sodium pyruvate: no NaHCO₃: no phenol red: yes HEPES: 25 mM L-glutamine: yes glucose: high	components glucose: high sodium pyruvate: yes NaHCO₃: no HEPES: no phenol red: yes L-glutamine: yes	components phenol red: yes L-glutamine: yes NaHCO₃: no HEPES: no glucose: high sodium pyruvate: no	components sodium pyruvate: no HEPES: 25 mM glucose: high NaHCO₃: yes L-glutamine: no phenol red: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
classic media, classical media, FBS, glucose enriched	classic media, classical media, FBS, glucose enriched	classic media, classical media, FBS, glucose enriched	classic media, classical media, serum supplement, FBS, HEPES, high glucose, pyridoxine

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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