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Citrate Synthase from porcine heart

Name: Citrate Synthase from porcine heart
Brand: SIGMA

liquid, ≥100 units/mg protein

Synonym(s):

Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

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About This Item

CAS Number:

9027-96-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

4.1.3.7 (BRENDA, IUBMB)

MDL number:

MFCD00130808

Specific activity:

≥100 units/mg protein

Biological source:

Porcine heart

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Properties

biological source

Porcine heart

Quality Segment

200

form

liquid

specific activity

≥100 units/mg protein

foreign activity

isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%

storage temp.

−20°C

Description

Application

Citrate Synthase from porcine heart has been used:

to inject newt egg for determining its importance in egg activation at fertilization
in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
to examine whether it can induce a [Ca²⁺] increase and egg activation in unfertilized eggs[1]

Biochem/physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H₂O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0.

Other Notes

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

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This Item	G9880	L7525	E2884
Sigma-Aldrich C3260 Citrate Synthase from porcine heart	Sigma-Aldrich G9880 Glutamic-Pyruvic Transaminase from porcine heart	Sigma-Aldrich L7525 L-Lactic Dehydrogenase from porcine heart	Sigma-Aldrich E2884 Esterase from porcine liver
specific activity ≥100 units/mg protein	specific activity ≥75 units/mg protein	specific activity ≥200 units/mg protein	specific activity ≥150 units/mg protein (biuret)
biological source Porcine heart	biological source Porcine heart	biological source Porcine heart	biological source Porcine liver
form liquid	form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
foreign activity isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%	foreign activity L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.05%	foreign activity glutamic-oxalacetic transaminase ≤0.1%, malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06%	foreign activity -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300

Storage Class

12 - Non Combustible Liquids

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