A4660

6-Thioguanine

Hybri-Max^™, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(s): 2-Amino-6-mercaptopurine, 2-Amino-6-purinethiol
• Empirical Formula (Hill Notation): C₅H₅N₅S
• CAS Number: 154-42-7
• Molecular Weight: 167.19
• UNSPSC Code: 12352207
• NACRES: NA.75
• PubChem Substance ID: 24890892
• EC Number: 205-827-2
• Beilstein/REAXYS Number: 157765
• MDL number: MFCD00233553
• Assay: ~98%
• Biological source: synthetic (organic)
• Form: lyophilized powder
• Solubility: 1 M NaOH: 50 mg/mL, clear to slightly hazy
• Storage temp.: room temp

Properties

• biological source: synthetic (organic)
• Quality Segment: 200
• grade: Hybri-Max^™
• sterility: γ-irradiated
• product line: BioXtra
• assay: ~98%
• form: lyophilized powder
• concentration: 50 ×
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• mp: ≥300 °C (lit.)
• solubility: 1 M NaOH: 50 mg/mL, clear to slightly hazy
• storage temp.: room temp
• SMILES string: NC1=Nc2nc[nH]c2C(=S)N1
• InChI: 1S/C5H5N5S/c6-5-9-3-2(4(11)10-5)7-1-8-3/h1H,(H4,6,7,8,9,10,11)
• InChI key: WYWHKKSPHMUBEB-UHFFFAOYSA-N
• Gene Information: human ... IMPDH1(3614), IMPDH2(3615)

Description

Application

Used in hybridoma formation applications as a selection agent.

Biochem/physiol Actions

Ribosylated and phosphorylated by the same pathway as natural purine bases; as the nucleotide, inhibits a variety of cellular processes involved in nucleic acid synthesis. Has a long history as an effective treatment of leukemia.

Preparation Note

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 30 μM 2-amino-6-mercaptopurine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Codes: H301
• Precautionary Statements: P301 + P310
• Hazard Classifications: Acute Tox. 3 Oral
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges