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A2289

Albumin, Tetramethylrhodamine isothiocyanate bovine

Name: Albumin, Tetramethylrhodamine isothiocyanate bovine
Brand: SIGMA

lyophilized powder

Synonym(s):

TRITC-albumin

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About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

NACRES:

NA.25

MDL number:

MFCD00130416

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥90% (Lowry)

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Impurities:

salt, free

Concentration:

≥90% protein (Lowry)

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Properties

biological source

bovine

Quality Segment

200

assay

≥90% (Lowry)

form

lyophilized powder

concentration

≥90% protein (Lowry)

extent of labeling

≥0.5 mol TRITC per mol albumin

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Albumin, Tetramethylrhodamine isothiocyanate has been used:

in photoinduced release assay of polyethylene beads
for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
in fluorescent correlation spectroscopy