This product is not suitable for use in MALDI TOF analysis. A bovine collagen standard that is suitable for MALDI TOF analysis is not available.
5006
Bovine Collagen Type I & III
from bovine hide, powder, suitable for cell culture, PureCol™
Synonym(s):
Collagen Type I, PureCol Collagen
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
Biological source:
bovine
Form:
lyophilized
Technique(s):
cell culture | mammalian: suitable
Assay:
99.9%
Pricing and availability is not currently available.
Product Name
PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture
biological source
bovine
sterility
sterile-filtered
assay
99.9%
form
lyophilized
packaging
pkg of 15 mg
technique(s)
cell culture | mammalian: suitable
binding specificity
Peptide Source: Elastin
, Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
−20°C
General description
PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.
It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
Application
PureCol™ has been used:
- in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids
- to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
- to coat culture plates for collagen degradation assay with breast cancer cell lines
Preparation Note
This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.
Legal Information
Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.
1 of 1
This Item | |||
|---|---|---|---|
| description Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture | description - | description from mouse(Natural), pack of 1 mg | description Ready-to-use ultrapure collagen type I protein for cell culture applications (20 mL). |
| sterility sterile-filtered | sterility sterile | sterility - | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell attachment: suitable | technique(s) - |
| biological source bovine | biological source bovine skin | biological source mouse (Natural) | biological source - |
| assay 99.9% | assay ≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE) | assay - | assay - |
| form lyophilized | form solid | form liquid | form solution |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
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Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hi, I am going to find bovine collagen standard (purified collagen) for using in MALDI TOF analysis. I wonder whether the collagen product 5006 is suitable in this regard. what is your other recommendation?
1 answer-
Helpful?
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How can this product be sterilised after reconstitution to 3 mg/ml?
1 answer-
The product is supplied sterile and should be reconstituted with 5ml of sterile 0.01M HCl. We would recommend filter sterilising the 0.01M HCl without collagen using a Millex syringe filter such as SLLG025SS (solvent resistant PTFE) or as it is only a weak acid solution then you could use cheaper SLGVR33RS (PVDF) sterile filters. It is not advisable to filter the collagen once in solution.
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Howlong is this product stable after reconstructiond and under which conditions should this stock solution be stored?
1 answer-
The long term stability of this product in solution has not been determined. However, historical data suggests that solutions stored protected from light, in amber glass or wrapped in foil, are stable at least 30 days when stored refrigerated at 2 - 8°C.
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