211842

Brilliant Black BN

Dye content 60 %

• Synonym(s): Food Black 1
• Empirical Formula (Hill Notation): C₂₈H₁₇N₅Na₄O₁₄S₄
• CAS Number: 2519-30-4
• Molecular Weight: 867.68
• Colour Index Number: 28440
• EC Number: 219-746-5
• MDL number: MFCD00078980
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852710
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 200
• composition: Dye content, 60%
• λmax: 407 nm (2nd); 570 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].CC(=O)Nc1ccc(c2cc(c(\N=N\c3ccc(\N=N/c4ccc(cc4)S([O-])(=O)=O)c5ccc(cc35)S([O-])(=O)=O)c(O)c12)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C28H21N5O14S4.4Na/c1-14(34)29-23-10-11-24(50(42,43)44)20-13-25(51(45,46)47)27(28(35)26(20)23)33-32-22-9-8-21(18-7-6-17(12-19(18)22)49(39,40)41)31-30-15-2-4-16(5-3-15)48(36,37)38;;;;/h2-13,35H,1H3,(H,29,34)(H,36,37,38)(H,39,40,41)(H,42,43,44)(H,45,46,47);;;;/q;4*+1/p-4/b31-30-,33-32+;;;;
• InChI key: GMMAPXRGRVJYJY-OXYXOBGMSA-J

Description

Application

Brilliant Black BN has been used:

• in micronucleus and comet assays to evaluate its genotoxic effect.
• as a quenching agent in calcium mobilization assay.
• as a redox indicator in microbiological inhibition bioassay using Bacillus megaterium to detect tetracycline and macrolides in milk.

Biochem/physiol Actions

[4-acetamido-5-hydroxy-6-[7-sulfonato-4-(4-sulfonatophenylazo)-1-naphtylazo], also known as food black 1, belongs to the group of azo dyes. Brilliant Black BN is widely used as a food dye and pharmaceutical excipient. In addition, it also acts as an allosteric modulator of the adenosine A1 and A3 receptors and is generally used within calcium mobilization assays to reduce extracellular fluorescence.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves