11467140001
Roche
DNA, MB-grade
from fish sperm
Synonym(s):
DNA
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About This Item
UNSPSC Code:
41106310
Recommended Products
biological source
fish (sperm)
Quality Level
grade
Molecular Biology
form
solution (ready-to-use)
mol wt
40-1,000 kDa
packaging
pkg of 500 mg
manufacturer/tradename
Roche
concentration
100 μg/mL
color
light yellowopalescent
solubility
water: miscible
storage temp.
−20°C
General description
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
Application
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Sequence
Chain Length 120 to 3,000 nucleotides
Physical form
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Preparation Note
Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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