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MAB2015

Sigma-Aldrich

Anti-Cartilage Proteoglycan Antibody, adult, clone EFG-4

ascites fluid, clone EFG-4, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

EFG-4, monoclonal

species reactivity

bovine, canine, pig, chicken, human, sheep, rabbit

should not react with

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)

Specificity

Recognizes the short peptides substituted with keratan sulfate side chains and within the core protein of proteoglycans in articular cartilages, chicken limb bud and cornea. Does not cross-react with human fetal cartilage proteoglycans.

Immunogen

Epitope: adult
Human adult cartilage proteoglycan.

Application

Anti-Cartilage Proteoglycan Antibody, adult, clone EFG-4 detects level of Cartilage Proteoglycan & has been published & validated for use in ELISA, RIA, WB, IH.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western blot: 1:1,00 - 1:200
Immunohistochemistry: 1:50 - 250
ELISA: 1:250 - 1: 2500
RIA: 1:20,000 - 1:40,000
Optimal working dilutions must be determined by the end user.I/

Physical form

Ascites fluid

Storage and Stability

Maintain at -20°C in aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniela A Frauchiger et al.
Tissue engineering. Part C, Methods, 25(10), 571-580 (2019-06-04)
Low back pain related to intervertebral disk (IVD) degeneration has a major socioeconomic impact on our aging society. Therefore, stem cell therapy to activate self-repair of the IVD remains an exciting treatment strategy. In this respect, tissue-specific progenitors may play
Adel Tekari et al.
Stem cell research & therapy, 7(1), 75-75 (2016-05-25)
The intervertebral disc (IVD) has limited self-healing potential and disc repair strategies require an appropriate cell source such as progenitor cells that could regenerate the damaged cells and tissues. The objective of this study was to identify nucleus pulposus-derived progenitor
C Manferdini et al.
Journal of tissue engineering and regenerative medicine, 10(5), 374-391 (2013-03-16)
Osteochondral lesions require treatment to restore the biology and functionality of the joint. A novel nanostructured biomimetic gradient scaffold was developed to mimic the biochemical and biophysical properties of the different layers of native osteochondral structure. The present results show
T Nukaga et al.
European cells & materials, 31, 95-106 (2016-01-28)
Transplantation of activated nucleus pulposus (NP) cells obtained by coculturing NP cells and bone marrow mesenchymal stromal cells having cell-to-cell contact has been shown to be effective in animal models and, more recently, in human clinical trials. If the NP
Raquel Vayas et al.
Cartilage, 12(3), 293-306 (2019-04-12)
The limits of the microfracture (MFX) treatment in terms of lesion size and long-term tissue functionality makes it necessary to investigate different alternatives to repair focal cartilage lesions. The present study aims at evaluating the efficacy of a minimally invasive

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