4876

p-Nitrophenyl Phosphate, disodium salt, hexahydrate

≥99% (HPLC), crystalline solid, alkaline phosphatase-based ELISA assay substrate, Calbiochem^®

• Synonym(s): p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, pNPP, 2Na
• Empirical Formula (Hill Notation): C₆H₄NO₆P · 2Na · 6H₂O
• CAS Number: 4264-83-9
• Molecular Weight: 371.14
• UNSPSC Code: 41116158
• NACRES: NA.77
• MDL number: MFCD00007319
• Assay: ≥99% (HPLC)
• Form: crystalline solid
• Storage condition: OK to freeze, protect from light

Properties

• Product Name: p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM).
• Quality Segment: 100
• assay: ≥99% (HPLC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• impurities: ≤0.005% p-nitrophenol (weight), ≤1% free phosphate (weight)
• color: light yellow
• solubility: water: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [Na+].[Na+].[P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-]
• InChI: 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChI key: VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description

General description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm^-1 M^-1 at 311 nm in 0.4 M Na₂CO₃.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate for alkaline phosphatase-based ELISA assays

Product does not compete with ATP.

Reversible: no

Preparation Note

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable