581301-U

Ascentis^® C18 (3 µm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

• Synonym(s): Ascentis RP18 HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® C18 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1 (Similar to Phenomenex Luna C18)
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 25% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 10 cm × 2.1 mm
• surface area: 450 m²/g
• surface coverage: 3.7 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: C18 (octadecyl) phase
• particle size: 3 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application

• Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: This study employs the Ascentis^® C18 HPLC column for the separation of proteotypic tryptic peptides, allowing the identification of human insulin and biosynthetic insulin analogues through multiplexed targeted unlabeled mass spectrometry. The method demonstrated high specificity and sensitivity, enhancing the accuracy of insulin analysis in pharmaceutical research (Qasem et al., 2019).


• Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben, and propylparaben in a topical cream preparation.: Utilizing the Ascentis^® C18 HPLC column, this research presents a novel stability-indicating method for the simultaneous assay of multiple compounds in a topical cream. The validated method offers robust stability assessment, critical for ensuring the quality and efficacy of pharmaceutical formulations (Byrne et al., 2014).


• HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: This paper describes the use of the Ascentis^® C18 HPLC column in HPLC-DAD and HPLC-ESI-MS/MS methods to profile metabolites in propolis extracts. The column′s performance in separating complex mixtures highlights its utility in natural product research and quality control (Pellati et al., 2011).


• Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: This study compares the performance of the Ascentis^® C18 HPLC column with other chromatographic columns for fast separations in pharmaceutical process development. The Ascentis^® column provided superior separation efficiency and speed, making it advantageous for high-throughput pharmaceutical analysis (Abrahim et al., 2010).

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable