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S9059

Sigma-Aldrich

Streptavidin−Gold from Streptomyces avidinii

~10 nm nominal (colloidal gold), buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD00132383
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.

conjugate

gold conjugate

Quality Level

200

form

buffered aqueous glycerol solution

concentration

~2.5 A520 units/mL

nominal

~10 nm (colloidal gold)

mean particle size

8-12 nm (monodisperse)

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.
Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.

Application

For detection of biotinylated compounds.
Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN2962
SLCH8187
SLCG1383
SLCF2555
SLCB9829

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Surface plasmon resonance (SPR) biosensor formats using gold nanoparticle or protein signal amplification for the sensitive assay of small molecules were developed using progesterone as a model compound. Progesterone was immobilized to a dextran surface in the Biacore biosensor through

Questions

1–2 of 2 Questions  

  1. Can you provide details on how streptavidin is conjugated to gold nanoparticles? Specifically, I'd like to know if there is any covalent binding involved or if it's a passive binding process.

    1 answer

    1. Product S9059 was manufactured using passive conjugation of the STP with 10 nm gold colloid. It does not involve covalent or linker chemistry. The protein binds to the gold colloid through amino acid residues, including charge attraction (lysine), hydrophobic attraction (tryptophan), and sulfur bonding (cysteine).

      Helpful?

  2. I want to know the streptavidin concentration in this stock. (e.g. 0.5 mg/mL)

    1 answer

    1. This information is reported as the protein concentration in the lot-specific Certificate of Analysis. A review of lots available in the last 7 years reveal a range of 11 - 11.6 ug/mL. Please see the link below to review a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/US/en/product/sigma/s9059#product-documentation

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