Skip to Content
Merck
All Photos(3)

Documents

PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Synonym(s):

Protease Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

shipped in

dry ice

storage temp.

−20°C

Application

Fluorescent Protease Detection Kit Video Protocol
Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Suitability

Detection limit: approx. 50 ng trypsin

Quantity

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Kit Components Only

Product No.
Description
  • Incubation buffer 25 mL
  • FITC-casein solution 5 mL
  • Assay buffer 200 mL
  • Protease standard (trypsin) 20 μg
  • FITC control 50 mg
  • Trichloroacetic acid (TCA) solution 30 mL

Signal Word

Danger

Hazard Statements

H314,H317,H334,H335,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Azoalbumin protease substrate

Sigma-Aldrich

A2382

Azoalbumin

Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles.
Sharma A, et al.
IEEE Transactions on Magnetics, 43, 2418-2420 (2007)
Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes
Kieloaho AJ, et al.
Soil Biology and Biochemistry, 96(10), 97-106 (2016)
Alejandro Soto-Gutiérrez et al.
Nature protocols, 2(2), 347-356 (2007-04-05)
This protocol describes a co-culture system for the in vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells. Differentiation involves four steps: (i) formation of embryoid bodies (EB), (ii) induction of definitive endoderm from 2-d-old EBs, (iii) induction of
Veronica Tjomsland et al.
European journal of immunology, 43(6), 1470-1483 (2013-03-26)
Induction of optimal HIV-1-specific T-cell responses, which can contribute to controlling viral infection in vivo, depends on antigen processing and presentation processes occurring in DCs. Opsonization can influence the routing of antigen processing and pathways used for presentation. We studied
Joseph S Rom et al.
Virulence, 12(1), 584-600 (2021-02-05)
Using the USA300, methicillin-resistant Staphylococcus aureus strain LAC, we previously examined the impact of regulatory mutations implicated in biofilm formation on protease production and virulence in a murine sepsis model. Here we examined the impact of these mutations in the
View All Related Papers

Articles

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Protocols

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service