N6009
Nerve Growth Factor-2.5S from murine submaxillary gland
lyophilized powder, suitable for cell culture
Synonym(s):
NGF-2.5S
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Product Name
Nerve Growth Factor-2.5S from murine submaxillary gland, NGF-2.5S, lyophilized powder, suitable for cell culture
biological source
mouse
Quality Level
form
lyophilized powder
potency
0.1-30 ng/mL EC50 (using PC-12 cells)
mol wt
26.5 kDa
packaging
pkg of 4X25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Ngf(18049)
General description
Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.
Application
Nerve Growth Factor-2.5S from murine submaxillary gland has been used:
- in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
- as a media supplement in the “process compartment” for microfluidic chamber studies
- to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression
Biochem/physiol Actions
Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.
Other Notes
2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.
Physical form
Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.
Analysis Note
The biological activity is measured using a 3-day MTT assay.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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