I5907
Pyrophosphatase, Inorganic from Escherichia coli
recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein
Synonym(s):
Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥90%
form
lyophilized powder
specific activity
≥800 units/mg protein
mol wt
hexamer subunit mol wt 20 kDa
storage temp.
−20°C
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General description
Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.
Application
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.
Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins. It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.
Biochem/physiol Actions
Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.
Other Notes
A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg2+ dependent. It is a relatively thermostable protein.
Unit Definition
One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.
Physical form
Lyophilized powder in Tris-buffered salts containing protease inhibitors
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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