GE17-5199-01
MabSelect™
Cytiva 17-5199-01, pack of 25 mL
Synonym(s):
MabSelect Column
About This Item
Recommended Products
ligand
recombinant protein A (E. coli)
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5199-01
matrix
highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
2-12
working range
3-10
capacity
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
Application
The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect™ is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.
The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect™ an excellent choice for purification of MAbs at process scale.
MabSelect™ is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect™ meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
- Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
- Low non-specific binding resulting in Low levels of impurities in the product eluate pool
- BioProcess medium supported for industrial applications and well-established in approved processes.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Protocols
This page shows how to pack Tricorn 10/100 columns with MabSelect or MabSelect SuRe from Cytiva.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
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