GE17-0408-01

HiTrap^® Chelating High Performance

Cytiva 17-0408-01, pack of 5 × 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: iminodiacetic acid
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-0408-01
• parameter: <4 mL/min flow rate; 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-14
• working range: 3-13

Description

General description

HiTrap^® Chelating HP is prepacked with Chelating Sepharose^™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Application

HiTrap^® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC^™ System

Features and Benefits

• For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
• Conveniently charge with your metal of choice.
• Iminidoacetic acid coupled to Sepharose^™ High Performance.
• Purifying Proteins with exposed histidine groups via metal ion complex formation.
• Simple operation with a syringe, pump or chromatographic system such as ÄKTA^® design or FPLC^™ System.
• Packed with Chelating Sepharose^™ High Performance
• Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids