G7882

L-Glutamic Dehydrogenase from bovine liver

Type III, lyophilized powder, ≥20 units/mg protein

• Synonym(s): L-GLDH, L-Glutamate:NAD[P]⁺ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver
• CAS Number: 9029-12-3
• Enzyme Commission number: 1.4.1.3 (BRENDA, IUBMB)
• EC Number: 232-848-4
• MDL number: MFCD00131461
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine liver
• Quality Level: 200
• type: Type III
• form: lyophilized powder
• specific activity: ≥20 units/mg protein
• UniProt accession no.: P00366
• storage temp.: −20°C
• Gene Information: cow ... GLUD1(281785)

Description

Application

L-Glutamic Dehydrogenase was used to catalyzes the conversion of isocitrate into a-ketoglutarate and carbon dioxide.[1]

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:

• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Packaging

Package size based on protein content

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Physical form

Contains citrate and potassium phoshate buffer salts.

Analysis Note

Protein determined by biuret

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves