D2178

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse

clone DAPK-55, ascites fluid

• Synonym(s): Anti-DAP Kinase
• MDL number: MFCD00282220
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: DAPK-55, monoclonal
• mol wt: antigen 160 kDa
• contains: 15 mM sodium azide
• species reactivity: human, monkey
• technique(s): immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:250 using COS-7 cell extract
• isotype: IgG1
• UniProt accession no.: P51397
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DAP(1611)

Description

General description

Monoclonal Anti-Death Associated Protein (DAP) Kinase (mouse IgG1 isotype) is derived from the DAPK-55 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Death-associated protein 1 (DAP1) gene is mapped to human chromosome 9q34.1. It belongs to the DAP family.

The gene is widely expressed in many tissues of embryonic and adult origin and gives rise to a single 6.3 Kb mRNA transcript. The protein consists of 1423 amino acids (160 kDa) and belongs to a family of calmodulin-dependent protein kinases. It contains 8 ankyrin repeats and 2 potential P-loop motifs apart from the kinase domain and the calmodulin regulatory domain.

Immunogen

recombinant human DAP kinase expressed in bacteria.

Application

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

DAP-kinase is a novel, structurally unique, human serine/threonine kinase identified as a positive mediator of interferon-γ-induced programmed cell death. It has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.

Death-associated protein 1 (DAP1) participates in the regulation of cell growth and death including that of cancer cells. DAP-kinase has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable