NCI-H69VCR/R

96042330, human lung, Not specified

• UNSPSC Code: 41106514
• eCl@ss: 32190102

Properties

• product name: NCI-H69VCR/R, 96042330
• biological source: human lung
• growth mode: Aggregates in suspension
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human small cell lung cancer, drug-resistant

Cell Line Description

NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (Sigma Catalogue number. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Drug-resistance studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 2.0 μg/ml vincristine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37ºC. Resuscitate in drug-free medium, add after growth has been established.

Other Notes

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