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Key Documents

R2C

89031606, rat testis, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

R2C, 89031606

biological source

rat testis

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Cell Line Origin

Rat testis Leydig cell tumour

Cell Line Description

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Culture Medium

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA;5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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