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B5655

Sigma-Aldrich

BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

Synonym(s):

BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ BCIP®/NBT, tablet

form

tablet

Quality Level

200

solubility

water: 10 mL, clear, yellow

storage temp.

−20°C

Related Categories

Application

SIGMAFAST BCIP®/NBT has been used as a substrate
  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts
  • for alkaline phosphatase staining in bone marrow stromal cells

Reconstitution

Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Aneela Yasmeen et al.
Molecular biotechnology, 58(12), 807-820 (2016-10-21)
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct
R Garg et al.
Human vaccines & immunotherapeutics, 13(12), 2894-2901 (2017-08-22)
Respiratory syncytial virus (RSV) is the most common cause of respiratory tract infections in both children and elderly people. In this study we evaluated the short- and long-term protective efficacy of a single intranasal (IN) immunization with a RSV vaccine
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
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Protocols

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

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