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Key Documents

453R-2

Sigma-Aldrich

p53 (EP9) Rabbit Monoclonal Primary Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP9, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (453R-24)
vial of 0.1 mL concentrate Research Use Only (453R-24-RUO)
vial of 0.5 mL concentrate (453R-25)
bottle of 1.0 mL predilute ready-to-use (453R-27)
vial of 1.0 mL concentrate (453R-26)
vial of 1.0 mL concentrate Research Use Only (453R-26-RUO)
vial of 1.0 mL pre-dilute Research Use Only (453R-27-RUO)
bottle of 7.0 mL predilute ready-to-use (453R-28)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (453R-28-RUO)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:200 (concentrate)

control

breast carcinoma ((breast ductal carcinoma in-situ)), colorectal carcinoma, ovarian carcinoma ((ovarian serous carcinoma)), urothelial carcinoma

shipped in

wet ice

storage temp.

2-8°C

visualization

nuclear

Gene Information

human ... TP53(7157)

General description

Anti-p53 tumor suppressor protein antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53, although significant accumulation of the mutant form of p53 protein due to longer half-life is the basis for the test using the IHC technique. Nuclear staining with this antibody has been shown in breast carcinoma, lung carcinoma, colorectal carcinoma, and urothelial carcinoma.

Quality

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Linkage

p53 Positive Control Slides, Product No. 453S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J K McKenney et al.
The American journal of surgical pathology, 25(8), 1074-1078 (2001-07-28)
Distinction of urothelial carcinoma in situ (CIS) from reactive atypia on the basis of morphology alone may be difficult in some cases. Because this distinction is therapeutically and prognostically critical, we attempted to determine if an immunohistochemical panel would help
David J Dabbs et al.
The American journal of surgical pathology, 37(7), e1-11 (2013-06-14)
Lobular neoplasia (LN) is a term that encompasses both lobular carcinoma in situ and atypical lobular hyperplasia. These lesions have been shown to constitute both risk indicators and nonobligate precursors of invasive breast cancer, they are relatively uncommon, and are
Diagnostic Immunohistochemistry (Masson Publishing), Page 701-Page 702 (2006)
O Caffo et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 2(9), 1591-1599 (1996-09-01)
p21 protein (p21) inhibitor of cyclin-dependent kinases is a critical downstream effector in the p53-specific pathway of growth control and can also be induced by p53-independent pathways in relation to terminal differentiation. We investigated p21 immunoreactivity in 261 breast carcinomas
Z S Zeng et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 12(10), 2043-2050 (1994-10-01)
This study was performed to determine the prognostic significance of p53 gene overexpression in a homogeneous group of node-positive colorectal cancer patients. Paraffin sections from the primary tumors in 107 colorectal cancer patients who had preoperative serum carcinoembryonic antigen (CEA)

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