Suspend 16 g of Phenol Red Broth Base in 1000 ml of distilled water. Heat to dissolve the medium completely. Dispense into tubes containing inverted Durham′s tubes and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Aseptically add a filter sterilized or autoclave sterilized carbohydrate solution to the base medium.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
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GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, 1 (1985)
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