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D8189

Sigma-Aldrich

Bis(1,3-dibutylbarbituric acid) trimethine oxonol

≥95% purity (HPLC), powder or solid

Synonym(s):

DiBAC4(3)

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About This Item

Empirical Formula (Hill Notation):
C27H40N4O6
CAS Number:
70363-83-6
Molecular Weight:
516.63
MDL number:
MFCD07437857
UNSPSC Code:
12171500
PubChem Substance ID:
329798857
NACRES:
NA.47

product name

Bis(1,3-dibutylbarbituric acid) trimethine oxonol, ≥95% (HPLC)

Quality Level

100

Assay

≥95% (HPLC)

form

powder or solid

color

orange to dark red

solubility

DMF: soluble
DMSO: soluble

ε (extinction coefficient)

≥90000 at 490-496 nm in methanol

fluorescence

λex 513-519 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CCCCN(C(N(CCCC)C1=O)=O)C(C1=C/C=C/C2C(N(CCCC)C(N(CCCC)C2=O)=O)=O)=O

InChI

1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3/b14-13+

InChI key

ATJCYSYHWGQAET-BUHFOSPRSA-N

Related Categories

Application

Bis(1,3-dibutylbarbituric acid) trimethine oxonol has been used for monitoring changes in the plasma membrane potential. It also has applications in high throughput screening.
Application in high throughput screening.

Biochem/physiol Actions

Bis(1,3-dibutylbarbituric acid) trimethine oxonolis a translational membrane potential dye that is redistributed in the cell as membrane potential changes.
Translational membrane potential dye that is redistributed in the cell as membrane potential changes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCV5935
MKCT9973
MKCS9018
MKCV4352
MKCS1692

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T Bräuner et al.
Biochimica et biophysica acta, 771(2), 208-216 (1984-04-11)
The usefulness of a new voltage-sensitive fluorescent dye, the membrane permeant negatively charged oxonol dye diBA-C4-(3)-, was evaluated by measuring the membrane potentials of BICR/M1R-k and L cells with glass microelectrodes and simultaneously recording the fluorescence of the stained cells.
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