230251

Eosin Y

75% (HPLC)

• Synonym(s): 2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43
• Empirical Formula (Hill Notation): C₂₀H₈Br₄O₅
• CAS Number: 15086-94-9
• Molecular Weight: 647.89
• Colour Index Number: 45380:2
• EC Number: 239-138-3
• MDL number: MFCD00036189
• UNSPSC Code: 12171500
• PubChem Substance ID: 24853789
• NACRES: NA.47

Properties

• Quality Level: 100
• Assay: 75% (HPLC)
• form: powder
• color: orange to very dark orange
• solubility: spirit: soluble
• λmax: 524 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br
• InChI: 1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H
• InChI key: DBZJJPROPLPMSN-UHFFFAOYSA-N

Description

General description

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Application

Eosin Y has been used as a reagent in the preparation of Nigrosin-Eosin (NE) stain and is used in hematoxylin and eosin (H&E) staining technique to visualize alginate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves