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MABT96

Sigma-Aldrich

Anti-Anillin Antibody, clone 5f3.1

clone 5F3.1, from mouse

Synonym(s):

Actin-binding protein anillin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5F3.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

General description

Anillin is a highly conserved multidomain protein that crosslinks multiple structural and regulatory elements including, but not limited to, F-actin, active myosin II, septins, and importin. It positively regulates normal cytokinesis; however, its molecular function within this context remains unclear. Failed cytokinesis results in binucleation, which can lead to genomic instability. In human cultured cells, anillin stabilizes TCA-fixable species of RhoA at the equatorial cortex, but its exact mode of action and subsequent physiological effects have yet to be determined. In some cancers, high levels of septins are shown to out-compete importin binding, thereby promoting the retention of Anillin in the cytoplasm.

Immunogen

GST-tagged recombinant protein corresponding to human Anillin.

Application

Anti-Anillin, clone 5F3.1 is an antibody targeting the Anillin protein, validated for use in WB & ICC.
Immunocytochemistry Analysis: 1 µg/mL of this antibody detected Anillin in the contractile rings of dividing HeLa cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in mimosine and nocodozole treated and untreated HeLa cell lysate.
Western Blot Analysis: 1.0 µg/mL of this antibody detected Anillin in 10 µg of mimosine and nocodozole treated and untreated HeLa cell lysate.

Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections.

Target description

~160 kDa observed. The calculated molecular weight of this protein is 124 kDa, but can be observed at ~160 kDa due to high glycosylation. Uniprot describes an isoform produced by alternative splicing at ~120 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mimosine and nocodozole treated and untreated HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eric Peterman et al.
Journal of cell science, 133(9) (2020-03-19)
During mitotic cell division, the actomyosin cytoskeleton undergoes several dynamic changes that play key roles in progression through mitosis. Although the regulators of cytokinetic ring formation and contraction are well established, proteins that regulate cortical stability during anaphase and telophase
Gabriella S Darmasaputra et al.
The Journal of cell biology, 223(8) (2024-05-10)
Binucleated polyploid cells are common in many animal tissues, where they arise by endomitosis, a non-canonical cell cycle in which cells enter M phase but do not undergo cytokinesis. Different steps of cytokinesis have been shown to be inhibited during

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