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MABS522

Sigma-Aldrich

Anti-PIKFYVE Antibody, clone R159.4.3C9

clone R159.4.3C9, from mouse

Synonym(s):

1-phosphatidylinositol 3-phosphate 5-kinase, Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

R159.4.3C9, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

General description

1-phosphatidylinositol 3-phosphate 5-kinase (EC 2.7.1.150; UniProt Q9Y2I7; also known as Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 29) is encoded by the PIKFYVE (also known as CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29) gene (Gene ID 200576) in human. PIKfyve is a class III lipid kinase that is responsible for phosphorylating phosphatidylinositol (PtdIns) and phosphatidylinositol 3-phosphate (PtdIns3P) of their inositol ring at the D-5 position to form PtdIns3P and PtdIns(3,5)P2 (phosphatidylinositol 3,5-bisphosphate), respectively. PIKfyve forms a stable ternary heterooligomeric complex with its regulator ArPIKfyve (encoded by the VAC14 gene) and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3 (encoded by the FIG4 gene), and both ArPIKfyve and Sac3 are required for maximal PIKfyve activity. PIKfyve is also known to interact with the Rab9 effector RABEPK and the kinesin adaptor JLP (encoded by SPAG9). By regulating endosomal trafficking, PIKfyve plays an important role in multiple biological processes, such as GLUT4 translocation, retroviral budding, autophagy, and neurodegeneration. Human PIKfyve is initially produced as a 2098-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. PIKFYVE gene mutation is linked to corneal fleck dystrophy (CFD) characterized by white flecks scattered in all levels of the stroma.

Specificity

Expected to react with all four spliced isoforms of human PIKfyve reported by UniProt (Q9Y2I7-1 through Q9Y2I7-4).

Immunogen

AviTag-6xHis-tagged recombinant protein corresponding an internal fragment of human PIKFYVE.
Epitope: Internal (N-terminal half).

Application

Anti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.
Research Category
Signaling
Research Sub Category
General Post-translation Modification
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PIKFYVE in 10 µg of HepG2 cell lysate.

Quality

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PIKFYVE in 20 µg of HL-60 cell lysate.

Target description

~240/50 kDa observed. 237,1 kDa (isoform 1), 50.21 kDa (isoform 2), 51.48 kDa (isoform 3), 61.60 kDa (isoform 4) calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ajit Roy et al.
Autophagy, 19(9), 2464-2484 (2023-02-22)
Although PIKFYVE phosphoinositide kinase inhibitors can selectively eliminate PIKFYVE-dependent human cancer cells in vitro and in vivo, the basis for this selectivity has remained elusive. Here we show that the sensitivity of cells to the PIKFYVE inhibitor WX8 is not
Sindhu Carmen Sivakumaren et al.
Cell chemical biology, 27(5), 525-537 (2020-03-05)
The PI5P4Ks have been demonstrated to be important for cancer cell proliferation and other diseases. However, the therapeutic potential of targeting these kinases is understudied due to a lack of potent, specific small molecules available. Here, we present the discovery

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